Characterization of two cis-acting elements, P1BS and W-box, in the regulation of OsPT6 responsive to phosphors deficiency

Abstract Phosphorus (P) deficiency is one of the major nutrient stresses restricting plant growth. The uptake P by plants from soil mainly mediated phosphate (Pi) transporters belonging to PHT1 family. Multiple genes diverse species have been shown be strongly up-regulated upon Pi starvation, however, underlying mechanisms for Pi-starvation-induced (PSI) up-regulation not well deciphered most transporter genes. Here, we reported a detailed dissection promoter activity PSI rice gene OsPT6 , using ?-glucuronidase (GUS) reporter gene. could drive GUS expression in both roots and blades grown under low P, but high P. Cis -acting element analysis identified copy P1BS motif two copies W-box promoter. Targeted deletion caused almost complete abolition induction response irrespective presence or absence motif, Four repeats fused CaMV35S minimal was sufficient induce responsive starvation. upstream (W1) did affect compared with full-length promoter, while targeted downstream (W2) motifs remarkably reduced rate Our results proposed that positively regulated at least elements, sole W-box, its W-box-mediated might highly dependent on motif.